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KMID : 0604520210470040297
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Journal of the Society of Cosmetic Scientists of Korea
2021 Volume.47 No. 4 p.297 ~ p.304
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Development of an In Vitro Pigmented Skin Model to Evaluate the Effectiveness of Whitening Functional Cosmetic Ingredients
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Kim Seol-Yeong
Lee Geon-Hee
Gwak Eun-Ji
Kim Su-Ji
Lee Su-Hyun
Lim Kyung-Min
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Abstract
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In this study, we prepared a pigmented skin model, KeraSkin-MTM for the in vitro evaluation of whitening agents.
For the purpose of complementing the existing mono-layer cell culture testing method, KeraSkin-MTM was produced through the co-culture of human skin-derived keratinocytes and melanocytes. The efficacy of four well-known whitening agents (arbutin, ascorbic acid, kojic acid, niacinamide) was evaluated in KeraSkin-MTM in order to assess its usefulness in assessing whitening efficacy. As a result, it was possible to observe additional details such as the distribution of melanin granules and melanin capping in each skin layer through KeraSkin-MTM, which was previously difficult to assess in the traditional 2D cell culture system. In addition, quantification through image analysis of KeraSkin-MTM allowed for a statistical analysis of the whitening effects. These results suggest that the KeraSkin-MTM can be used as a new evaluation method of evaluating whitening efficacy, as well as complement the traditional total melanin content and tyrosinase inhibition assays.
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KEYWORD
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whitening, alternative test method, pigmented human skin model, KeraSkin-MTM
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